Applications of Prominence RF-20AXS Fluorescence Detector(Part 3) Analysis of Saccharides Using Post-Column Derivatization System

Introduction

The Shimadzu Prominence reducing sugar analysis system is a post-column derivatization system; after separation of saccharides by the column, an arginine/boric acid reagent solution is continuously added to the column eluent to convert the saccharides to fluorescent derivatives for detection. This system allows detection of saccharides at high sensitivity and with excellent selectivity, furthermore with the new Prominence RF-20A_XS, even higher sensitivity is achieved. Additionally, the temperature controlled flowcell in the RF-20A_XS allows highly reliable analysis that is unaffected by ambient temperature fluctuations. Here we present some examples of analyzing saccharides using the reducing sugar analysis system (anion exchange mode) with the Prominence RF- 20A_XS.

April 22, 2010 GMT