Analysis of Nivalenol and Deoxynivalenol in Wheat Using Prominence-i

Introduction

Nivalenol and deoxynivalenol (DON, vomitoxin) are types of mycotoxins produced by Fusarium fungi. In Japan, the provisional reference value for deoxynivalenol was set at 1.1 ppm in May, 2002 (Notification No. 0521001 issued by Department of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare of Japan). Previously, in Application News No. L362, the analysis using an ultra high-performance LC system was introduced, but here, referring to the test method for deoxynivalenol specified in the Notification No. 0717001 (issued by Department of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare of Japan, in July 2003), a washing process for the analytical column is included. The detector component of the new Prominence-i integrated high-performance liquid chromatograph incorporates a temperature control function for both the flow cell component and the optical system. Here, good repeatability was obtained despite the susceptibility of UV detection in the short wavelength region due to environmental temperature fluctuations.

February 20, 2015 GMT