Analysis of Compounds Containing a Phosphate Group Using the New MastroTM High Pressure-Resistance Stainless Steel- Free LC Column

Introduction

Substances containing phosphate groups or metal chelates are often found in pharmaceuticals and metabolites. When subjected to HPLC analysis, these compounds are known to adsorb to metallic surfaces in the HPLC system flow line, thereby causing adverse peak tailing in their chromatograms. The typical remedy for this has been to add chelating agents and salts to the mobile phase, but the use of such nonvolatile eluents is not applicable when using an LC/MS as the detector. Here, we examined the effects of different flow line materials on the peak shape of coordination compounds. Also, using a formic acid mobile phase, which is commonly used in LC/MS analysis, we examined the inhibiting effect on metal adsorption of coordination compounds that is provided with MastroTM high pressure-resistance stainless steel-free analytical column.

May 22, 2013 GMT