Analysis of Cresol Positional Isomers by Using Shim-pack™ GIST Phenyl Column

Introduction

Reversed phase chromatography (RPC), which is widely used in high performance liquid chromatography, is applicable to diverse compounds. The packing materials for columns used in RPC are substances that bond chemically with alkyl groups and other functional groups as a simple substance of silica gel. The octa decyl group (C18) and the octyl group (C8) may be mentioned as representative examples. The ODS (Octa Decyl Silyl) column is frequently used as the first choice when studying separation conditions, but separation of isomers is inadequate in some cases. Phenyl columns show different separation behavior from ODS columns. Due to the phenyl skeleton, in addition to the hydrophobic interaction, the π-π interaction also contributes to separation in the phenyl column, resulting in higher selectivity for aromatic compounds. This article introduces an example of analysis of positional isomers of cresol by using a phenyl column that displays different separation characteristics from ODS columns among RPC columns.

September 27, 2019 GMT