Verification of Quantitativity and Confirmation of Molecular Weight of Oligonucleotide therapeutics Using LCMS™-8060

Introduction

Oligonucleotide therapeutics are synthetic oligonucleotides which demonstrate medical efficacy by bonding with target genes or target proteins that cause various diseases. This article introduces an example of an analysis of a 20 base 2'-MOE modified oligonucleotide using an LCMS-8060 triple quadrupole mass spectrometer. In preparation of the calibration curve in the MRM mode, linearity was confirmed in the range of 1 to 300 ng/mL, and the molecular weight was confirmed by deconvolution from the multivalent ion mass spectrum obtained in the scan mode.

November 20, 2020 GMT