Development of Multi-target Screening Method for Toxicological Compounds in Blood Samples on A Fully-automated CLAM-LC-MS/MS Platform

Introduction

Multi-target screening by LC/MS/MS has been widely adopted in detection and quantitation of drugs of abuse (DoA) in forensic investigation and toxicological research. A wide range of targets screened includes illicit drugs, narcotics, psychotropics, antipsychotics, pharmaceuticals and other toxic compounds in urine, serum/plasma and whole blood samples. Sample preparation is often a bottleneck due to the tedious steps. It is also a factor responsible for inaccurate or false negative results. We describe a solution by using an automated sample preparation module CLAMTM -2000 coupled to LC/MS/MS system (LCMS^TM-8060) for multi-target screening of 61 drugs in whole blood. A ready-to-use method package Rapid Toxicology Screening was used to set up the screening method with human whole blood (frozen) spiked sample without efforts in LC and MRM method development.

November 25, 2020 GMT