Peptide Mapping of Monoclonal Antibody (mAb) Using LCMS™-9030 (Q-TOF) Mass Spectrometer with a Shim-pack™ GISS-HP Column

Introduction

Monoclonal antibody (mAb)-based biotherapeutics are emerging as one of the fastest-growing categories of biologic drugs being developed today. Peptide mapping is a key analytical method for quality assurance of mAb products. It is employed for the elucidation of primary structure of mAb biosimilars. In the previous application news AD-0176 [1], we had developed a mAb peptide mapping workflow by using both Nexera TM Bio UHPLC and LCMS-9030 (Q-TOF) systems, yet the method is time consuming, taking 2 hours for each analysis. In the present report, we aim to optimize the workflow to reduce the running time and maintain the 100% peptide sequence coverage on Shimadzu LCMS-9030 (Q-TOF) mass spectrometer.

February 4, 2020 GMT