Evaluation of Undifferentiated State of Human iPS Cells Using C2MAP™ Cell Culture Media Analysis Platform

Introduction

Development of technologies for the preparation and supply of iPS cells with high quality and a large amount is an essential requirement for commercialization of regenerative medicine. Conventionally, manual techniques such as Gene Expression analysis had been used to evaluate the status of cell differentiation, but because these are invasive techniques that cause cell disruption, they were generally applied as evaluation methods after culturing was completed. We developed the C2MAP cell culture media analysis platform using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the aim of establishing a non-invasive cell status evaluation method based on component analysis of the cell culture supernatant.

April 21, 2020 GMT