Quantitative Analysis using Second-order Derivative Spectrum

Introduction

Derivative spectral analysis is often used for the peak identification due to its advantages in differentiating closely adjacent absorption peaks, identifying weak absorption peaks obscured by sharp peaks, and identifying wavelength at maximum absorbance for broad spectra. Because the derivative values and concentration levels have a linear relationship, quantitation is easily performed even when background absorption exists. This Application News presents an example of measurement using derivative spectrum with a UV-Vis spectrophotometer.

March 14, 2013 GMT