Quantitation of DNA Using Nano Stick

Introduction

An ultraviolet-visible spectrophotometer can be used for quantitation and purity confirmation of nucleic acids and proteins. As such substances are typically available only in very small quantities, a cell that can accommodate trace level samples is required for measurement. Up to now, an ultra-micro black cell has been used for measurement of 25 μL sample volumes. Here, we introduce an example of trace-level measurement of nucleic acids using a SCINCO Nano Stick microliter cuvette, which permits measurement of 2 μL samples.

October 27, 2014 GMT