Detection of Cysteine/Cystine Using Protein Sequencer

Introduction

The main approach in protein analysis at present is proteomic analysis, in which proteins are identified by using a mass spectrometer and search engine employing a genome database. The proteins expressed in a living body have various functions as a result of post-translational modification, and the theoretical mass numbers of the precursor proteins and maturation proteins are different in some cases. Identification of amino acid sequences with a mass spectrometer without using a database is both complex and difficult. In contrast, the conventional protein sequencer method using Edman degradation provides highly reliable amino acid sequence results, while enabling easy identification of amino acid sequences even the database is inadequate. This article introduces an example of an amino acid sequence analysis of a sample containing cysteine and cystine by using the Shimadzu PPSQ™-50A protein sequencer systems (isocratic system and gradient system)

May 12, 2020 GMT