Simultaneous Detection by MS, PDA and Fluorescence Detectors with LCMS-2010A System

Introduction

To improve the reliability of qualification in LC-MS analysis, light absorption (UV) detector and photodiode array (PDA) detectors, which are often used in HPLC analysis, are adopted in addition to mass spectrometers. Using the high-performance liquid chromatograph mass spectrometer LCMS-2010A and its control/processing software LCMSsolution, three phenolic antioxidants BHA (Butylated hydroxyanisole), NDGA (Nordihydroguaiaretic acid) and TBHQ (tert-butyl hydroquinone) were analyzed. Detection was made using fluorescent, PDA and MS detectors. The flow line was branched using a splitter after the column for a flow ratio of 8:2 for fluorescent and PDA/MS detectors. Quasi-molecular ions ([M-H]-) were detected for all components. Using LCMSsolution allows simultaneous acquisition of data from fluorescence, PDA and MS detectors, improving the qualification reliability.

October 31, 2003 GMT