Analysis of Modification Site of Chemically Modified Antibody Using MALDImini™-1 Compact MALDI Digital Ion Trap Mass Spectrometer

Introduction

Antibody drug conjugates (ADC), which appeared in the 2000s, are a new class of anti-cancer drugs in which an antibody is bound to a cytotoxic drug. Because they combine the high substrate specificity of the antibody and the effect of a low- molecular drug, ADC are expected to be more effective anti- cancer drugs than the conventional low-molecular drugs. When a different compound is bound artificially to a protein, as in the case of ADC, the binding degree of that compound and its binding site become one of the critical quality properties. Therefore, as reported in the example in this article, a pseudo ADC was created by artificially binding a low-molecular compound to a standard research antibody, and was then analyzed using a MALDImini-1 compact MALDI digital ion trap (DIT) mass spectrometer.

June 24, 2019 GMT