Quantitation of Nucleic Acids

Introduction

Ultraviolet-visible (UV-VIS) spectrophotometers are used in quantitative and qualitative analysis of substances in many fields. Purity confirmation and quantitation of nucleic acids, proteins, and other substances are also performed in the life sciences, but measurement at the trace level is demanded, as only small samples are available in many cases. This article introduces an example of quantitation of nucleic acid with a Shimadzu UV-1900 UV-VIS spectrophotometer with two type of cells (TrayCell^TM, Hellma Analytics and Nano Stick, SINCO) which enable measurement of sample quantities of several μL. The Biomethod Mode of the UV-1900 is also introduced briefly.

May 30, 2018 GMT