Development and Validation of Non-derivatization LC/MS/MS Method for Fast Determination of Proteinogenic Amino Acids in Fish

Introduction

Amino acids (AA) play a central role as building blocks of proteins and intermediates in metabolism that helps to maintain health and vitality. Fish is a source of proteins rich in essential amino acids which cannot be synthesized in human. Conventional methods for analysis of AA are based on HPLC-RF using pre- or post-column derivatization which runtime is normally from 30 mins to 2 hours. Thereby, there are demands in simpler and faster analysis without derivatization method for AA determination in various food matrixes. This study focuses on development and validation of a novel non- derivatization MRM-based method for fast quantitation of total amino acids in hydrolyzed fish using an Imtakt column. The method performance was validated and compared with UHPLC-RF method.

December 6, 2019 GMT