LC/MS/MS Method with HILIC Separation for Quantitation of Fosfomycin in Human Plasma

Introduction

Fosfomycin (FOS) is an old and broad-spectrum antibiotic drug manufactured since 1970s. It is mainly used for treatment of urinary tract infections (UTIs). However, the development of bacterial resistance occurs frequently, making fosfomycin unsuitable for treatment of severe infections in the past. Recently, use of fosfomycin formulations was approved in several countries, because it was found to be active against many multidrug-resistant (MDR) pathogens. Quantification of fosfomycin in human plasma may provide insight into its pharmacokinetics characteristics, which is crucial for current therapy modification. Therefore, a reliable analytical method is needed for determination of fosfomycin in biological samples. In this study, a LC/MS/MS method with HILIC chromatography was developed and used for quantification of fosfomycin, a small and highly- hydrophilic antibiotic, in human plasma.

April 17, 2020 GMT