Analysis of Chlorpromazine in Milk and Chicken Egg Extracts using Triple Quadrupole LC/MS/MS

Introduction

Chlorpromazine hydrochloride is used as a tranquilizer (pharmaceutical). At the same time, the use of veterinary medicines that have chlorpromazine as an active constituent is prohibited on animals to be used for food, and those which produce milk, eggs, etc. to be shipped for food. (Ministry of Agriculture, Forestry and Fisheries Ordinance No. 44, 2013) In addition, in the Positive List system, chlorpromazine is classified as a substance which must not be contained in food, and the LC/MS method has been cited as the method for testing for it in the "Standards for Food, Food Additives, etc." (Ministry of Health and Welfare Notification No. 370, 1959). However, this test method cannot be applied to all livestock and seafood, and it is being reviewed because it may not be possible to obtain good analysis results depending on the food. In March 2019, the Pharmaceutical Affairs and Food Sanitation Council (food sanitation subcommittee, agricultural chemicals and veterinary medicines group) reported a consultation document (Ministry of Health, Labour and Welfare Notification 0220-4) on a new chlorpromazine test method whose development has been completed. In this article, we present an example analysis of chlorpromazine in milk and chicken eggs in accordance with the test method described in the consultation document.

December 6, 2019 GMT