A Targeted Phospholipid Profiling Approach with PCA for Beans and Milk Using a Ready-to-Use MRM Method Package on LC/MS/MS

Introduction

Phospholipid profiling is usually performed by untargeted screening on GCMS and LC-TOF which involves extensive method development and data analysis work, due to complexity in structures. Shimadzu has developed an MRM method package for rapid profiling of phospholipids, covering 867 main components of various types of phospholipid with C14~C22 fatty acids. In this study, this ready-to-use MRM method package was utilized for targeted screening of phospholipids in beans (Glycine max, Vigna radiata) and milk products without use of any standards successfully. By coupling with TQ-MS and PCA, this study aims to establish a rapid and highly sensitive phospholipid profiling approach on LC/MS/MS for food classification and authentication.

January 16, 2020 GMT