Simultaneous Analysis of Five Vitamers of B3 and B6 in Banana using LC/MS/MS

Introduction

Vitamin B3 and B6 are water-soluble vitamins that are essential micronutrients to maintain good health. Vitamin B3 helps to lower cholesterol level and improves skin function while vitamin B6 plays an important role in the nervous system. These two vitamins are mainly obtained from meats, vegetables and fruits. Banana is an excellent source of both vitamins. Many of the available methods analyse vitamins B3 and B6 separately using HPLC coupled with fluorescence detector which can be timeconsuming. Here, we developed a sensitive LCMS/MS method to simultaneously analyse 5 vitamers of B3 and B6 namely, nicotinic acid, nicotinamide, pyridoxamine, pyridoxal and pyridoxine using LCMSTM-8045 with acid hydrolysis as the sample extraction method.

January 12, 2021 GMT