Fully automated quantification of Meropenem, Tazobactam, Piperacillin and Dexamethasone in plasma

User Benefits

- Simultaneous quantification of a corticosteroid and 3 antibiotics - Rapid analysis within 5 min and fully automated sample preparation - Sufficient repeatability and robustness

Introduction

This application describes the analysis of one corticosteroid Dexamethasone and 3 antibiotics Tazobactam, Meropenem and Piperacillin. Dexamethasoneis a synthetic glucocorticoid hormone. It has an anti-inflammatory and immunosuppressive effect. It is widely used for reducing swelling associated with tumors, and treat eye inflammation. Dexamethasone can help treat allergic reactions and be used in cancer treatments too1. More recently, studies have demonstrated the effectiveness of dexamethasone in the treatment of COVID-19, mainly for people with acute respiratory distress syndrome (ARDS). Corticosteroid therapy reduced the risk of all-cause mortality (risk ratio 0.75; 95% CI, 0.59–0.95). In order to avoid the risk of bacterial overgrowth, which is more common with corticosteroid treatment, an antibiotic therapy is often combined with it. The following method allows the simultaneous quantification of corticoid and antibiotic in biological samples with a fully automated sample preparation due to the use of CLAM system. By simplifying uncapping blood collection tubes (or sample cups), placing the pretreatment vials in the system, and requesting analysis, the system performs all other process steps automatically, from pretreatment to LCMS analysis. This fast analytical method (=5min) and its automatic and overlapped sample preparation allows to treat a large number of samples in a minimum of time. The sensitivity of the LCMS-8060 allows to sweep samples with an analysis dynamic range from 1 to 100 ng/mL.

March 31, 2021 GMT