Simplifying Natural Product Analysis with Data-Independent Acquisition Workflow

User Benefits

- Data-independent acquisition mode on Shimadzu LCMS-9030 Q-TOF enables comprehensive coverage of chemical compounds for natural product research - LabSolutions LCMS simplifies the setup of data-independent acquisition mode - LabSolutions Insight Explore delivers seamless data-processing tools to analyze data-independent acquisition data

Introduction

Natural products are intensively studied because they are a rich source of chemical diversity capable of delivering pharmacological benefits. High-resolution mass spectrometry (HRMS) instrument such as quadrupole time-of-flight (Q-TOF) is frequently used to aid the identification of unknown natural products. In addition, natural product chemists are equally concerned with obtaining a full coverage of chemical compounds to reduce false-negative identifications. The data-independent acquisition (DIA) method performed on the HRMS instrument is thus designed to maximize the unknown compounds coverage and their identification. DIA method acquires MS/MS scans systematically without prior knowledge of target precursor information. Due to the independence between precursors and fragment ions in DIA analysis, it is necessary to have a powerful data analysis solution that allows a rigorous and exploitable analysis of data. In this article, we will demonstrate the acquisition and data processing workflow solutions for DIA method on Shimadzu LCMS-9030 Q-TOF. A traditional herb sample, Aconitum carmichaeli, will be used to illustrate the workflow.

June 21, 2021 GMT