A Demonstration of HRMS with CE Spread Function for Identification of Cytotoxic Contaminant Present in Marine Bacteria Extract for Natural Product Discovery

User Benefits

- The flexible CE spread feature on LCMSTM-9030 Q-TOF enriches the fragment ions of precursors, which enables to enhance the identification reliability of compounds. - The LabSolutions Insight Explore s/w facilitates the identification workflow by automatically formula prediction, databasev search and fragment annotation for easy structural elucidation.

Introduction

Natural products from marine bacteria are the important source of new therapeutics in drug discovery in recent years. However, raw materials such as crude microbial extracts from where these natural products come from may contain contaminants that can cause harm to human health or give a false positive result in cytotoxicity assays. Hence, detection and identification of bioactivity compounds and contaminant analysis are crucial steps towards resolving suspected contamination of raw materials. Among various analytical instruments and technologies, mass spectrometry with fragmentation has been used to provide evidences in structural elucidation and identification of unknown compounds by GC-MS and LC-HRMS for decades. In this article, an easy-to-use and reliable approach for identification of compounds by LC-Q-TOF is described.

September 24, 2021 GMT