Analysis of Ornithine in Bunashimeji (Beech mushroom) Using Triple Quadrupole LC/MS/MS

User Benefits

- The LC/MS/MS method enables highly sensitive analysis (sample conversion concentration: 50 mg/kg or less) of ornithine without derivatization. - In addition to high sensitivity analysis, high speed analysis (13 min) is also possible with this system.

Introduction

Ornithine is a type of free amino acid that exists in large amounts in shijimi (Corbicula leana), etc., and has been widely recognized as an effective component for improving liver function in recent years. Ingestion of ornithine is expected to have various positive health benefits, including recovery from fatigue, improvement of the quality of sleep, and enhanced secretion of growth hormones. Ornithine plays an important role in supporting the functioning of the liver, which metabolizes ammonia, a harmful substance formed in the human body. This article introduces an example of an analysis of the edible mushroom known as bunashimeji (beech mushroom), which is said to have an ornithine content at least 5 times greater than that of the shijimi. The analysis was carried out in accordance with the Japanese Agricultural Standard (JAS0016) * “Determination of the ornithine in mushroom (Hypsizygus marmoreus) ― Highperformance liquid chromatographic method,” which was announced as Ministry of Agriculture, Forestry and Fisheries Notification No. 445 on March 31, 2021, using a Shimadzu LCMS8060NX. * Shimadzu Corporation participated in the interlaboratory collaboration in the establishment of this JAS standard.

November 26, 2021 GMT