Method for the determination of 346 Residual Pesticides in Milk using LCMS-8045 and GCMS-TQ8040 NX

User Benefits

- A modified QuEChERS extraction procedure has been employed for quantifying the pesticides at the desirable concentration levels using Ultra-fast technologies of LCMS-8045 and GCMS-TQ8040 NX. - Shorter run time of analysis increases the productivity and throughput of the LC-MS/MS and GC-MS/MS system. - Method using lower injection volumes and lower flow rates increases a column lifetime and a stability of assay over longer analytical periods.

Introduction

Milk is an important food in the diet, especially for infants and children. The presence of any contamination in milk is a common food safety concern. Hence great efforts have been taken throughout the dairy industry to ensure the safety of milk. One of the main classes of contaminants in milk is pesticides, which can come from animals ingesting contaminated feed or water. The maximum residue limits for pesticides in milk are often much lower than for general fruits and vegetables. Therefore, the analysis of pesticides in milk requires a sample preparation method for better matrix removal and analytical instrument methods for increased sensitivity. The aim of this study is to develop a simple and efficient workflow for determining a wide range of pesticides that are broadly controlled in milk worldwide. Based on these requirements, Shimadzu Application Development Center (ADC) has developed and validated a multiresidue method which is a simple, sensitive and high throughput multiclass method to determinate 346 pesticides in milk by using LCMS-8045 and GCMS-TQ8040 NX. The multi-residue extraction was performed with modified QuEChERS method for simultaneous quantification of 223 pesticides by LC-MS/MS and 167 pesticides by GC-MS/MS. Out of these, 44 pesticides were common and analyzed by both the techniques.

November 14, 2025 GMT