Method for the determination of 182 Residual Pesticides in Milk using LCMS-8045 and GCMS-TQ8040 NX

User Benefits

- A modified QuEChERS extraction procedure has been employed for quantifying the pesticides at the desirable concentration levels using Ultra-fast technologies of LCMS-8045 and GCMS-TQ8040 NX. - Method with shorter run time can contribute to achieving high throughputs in testing laboratories.

Introduction

Milk is an important food in the diet, especially for infants and children. The presence of any contamination in milk is a common food safety concern. Hence great efforts have been taken throughout the dairy industry to ensure the safety of milk. One of the main classes of contaminants in milk is pesticides, which can come from animals ingesting contaminated feed or water. The maximum residue limits for pesticides in milk are often much lower than for general fruits and vegetables. Therefore, the analysis of pesticides in milk requires a sample preparation method for better matrix removal and analytical instrument methods for high sensitivity. The aim of this study is to develop a simple and efficient workflow for determining a wide range of pesticides that are broadly controlled in milk worldwide. Based on these requirements, Shimadzu Application Development Center (ADC) has developed and validated multi-residue method which is a simple, sensitive and high throughput to determinate of 182 pesticides in milk using LCMS-8045 and GCMS-TQ8040 NX. The multi residue extraction was performed with modified QuEChERS method for simultaneous quantification of 113 pesticides by LC-MS/MS and 92 pesticides by GC-MS/MS. 23 out of these pesticides were common to both techniques. Out of 66 pesticides listed under FSSAI, 61 pesticides were covered in this study. These compounds are marked with # in the summary results’ table. From the remaining 5, Flubendiamide and Chlorothalonil are required to be analysed at higher concentration levels than the range selected for this work. Remaining 3 pesticides i.e., Glufosinate, Paraquat and Triacontanol require single residue methods for analysis.

January 14, 2022 GMT