Analysis of residual pesticides in Carrotoleoresin using GCMS-TQ8040 NX and LCMS-8045

User Benefits

- The method involves study of LOQ on both GC-MS/MS and LC-MS/MS, based on validation parameters like linearity, recovery, repeatability and within-laboratory reproducibility. - A modified QuEChERS extraction procedure has been employed for quantifying the pesticides at trace levels in complex matrix like Carrot-oleoresin using Ultra-Fast technologies of LCMS-8045 and GCMS-TQ8040 NX. - LCMS Method Package for Residual Pesticide Ver.3 and GCMS Smart Pesticides Database^TM Ver.2 from Shimadzu Corporation enables ease of optimizing instrumental method.

Introduction

Carrot is often contaminated with pesticide residues, due to the application of various chemicals for the control of ectoparasites, insects and pests. Carrot is used to prepare or extract natural colour additives in the form of oleoresin. Oleoresin is produced by solvent extraction of carrot powder or dehydrated carrot using a suitable organic solvent. Alternatively, the oleoresin can be recovered by steam distillation followed by solvent extraction. During the extraction process, the pesticide residues get concentrated in these oleoresins. The carrot oleoresins are used as colour additives in food products, cosmetics, nutraceutical and pharma drugs. Hence quantitation of residual pesticides in carrot oleoresin becomes very important. As the oleoresin is a complex matrix for extraction, it is required to develop a rugged, sensitive and easy method for residual pesticide analysis.

January 14, 2022 GMT