Simultaneous Determination of Functional Components in Coffee

User Benefits

- Simultaneous analysis of highly polar compounds from acidic to basic is possible. - The peaks of the compounds with basic group that have generally poor retention in reversed phase columns can be retained, and stable analysis can be achieved.

Introduction

Recently, coffee drinking has been reported to be effective in preventing or improving dementia and lifestyle-related diseases such as diabetes and cancer. It has also been found that coffee components such as pyrocatechol and trigonelline contribute to these effects. However, trigonelline, a compound with basic group, is difficult to analyze due to poor retention on a C18 column. Therefore, a PFPP column that has pentafluorophenylpropyl as the stationary phase was used for the analyses in this study. This column is expected to provide a unique separation selectivity as a result of π–π interaction and dipole-dipole interaction in addition to hydrophobic interaction. This article describes simultaneous determination of functional components in coffee using a Shim-pack Scepter^TM PFPP.

March 1, 2022 GMT