Determination of Functional Component in Agricultural Product: β-cryptoxanthin in Mandarin Orange by HPLC Method

User Benefits

- Quantitative determination of functional component of β-cryptoxanthin (BCR) in fresh mandarin orange samples using an i-series LC-2050C with a high sensitivity UV detector and an excellent ODS column for separation. - High throughput, accurate and reliable method to measure the functional nutrient in fresh fruits.

Introduction

Satsuma Mandarin is listed as a functional food in Japan, which can be recognized by Functional Food Claims (FFC), a new regulatory system of health claim introduced in Japan in 2015. The Japanese Agricultural Standards (JAS) are established to support the FFC new functional food system. β-cryptoxanthin can be found in fruits such as mandarin orange, papaya, and mango. β-cryptoxanthin has several beneficial effects on human health, such as acting as an antioxidant on scavenging free radicals from our body for preventing cancer, being a precursor to vitamin A on improving human health vision, reducing the signs of ageing, etc. In this application news, a HPLC method associated with the JAS 0003 reference method is described for quantitation of BCR in fresh mandarin orange. The sample preparation and HPLC analysis were performed in accordance with the JAS method 0003.

March 10, 2022 GMT