A LC-ESI-Q-TOF Method for Identification and Relative Composition Analysis of Triacylglycerols in Tropical Oil - (1) Coconut Oil

User Benefits

- A fast and direct LC-ESI-Q-TOF method was established for identification of TAGs in coconut oil and determination of the relative composition of various TAGs with different fatty acids. - A data analysis procedure was set up for unambiguous identification of individual TAG molecule from accurate mass MS and MS/MS spectra using the LabSolutions Insight Explore s/w.

Introduction

Tropical oils, namely coconut oil and palm oil etc., are widely used in tropical countries for cooking and other purposes for long history. Triacylglycerols (TAGs) are the major form of dietary lipid in edible oils including tropical oils. Characterization of TAGs in oils from types to composition is very essential for the evaluation of nutritional values and health impact etc. Traditionally, TAG composition is determined by HPLC-RID method, but identification relies on indirectly GC/MS method via hydrolyzing first to fatty acids, following by derivation and GC/MS analysis. In recent years, direct analysis methods by LC-MS/MS and SFC-MS/MS have been used for quick identification of TAGs. In this application note, we introduce a newly-established LC- ESI-Q-TOF method for quick identification of TAGs in coconut oil and determination of their relative composition simultaneously.

March 30, 2022 GMT