Measuring Choline and its Metabolites in Human Plasma Using an LC/MS/MS System with Fully Automated Sample Preparation Module

User Benefits

- Capable of quantifying choline, TMA, and TMAO levels in biological samples - Performs time-consuming sample preparation automatically. - Eliminates the need for manual sample preparation and reduces variability in quantitative results due to manual operations.

Introduction

It is known that some of the choline ingested in food is partly metabolized to trimethylamine (TMA) by intestinal bacteria and subsequently metabolized to trimethylamine N-oxide (TMAO) by enzymes in the liver. Reports have noted that TMAO is associated with cardiovascular diseases such as arteriosclerosis and that it is useful in predicting the prognosis of heart failure , As a result, TMAO is now attracting interest as a new biomarker. This article describes a system for simultaneous analysis of choline and choline metabolites in human plasma using an LC/MS/MS system with fully automated sample preparation module that automatically performs deproteination of biological samples and adds internal standards.

April 5, 2022 GMT