High-Speed Simultaneous Analysis of Amino Acids by Pre-column Derivatization Using Automatic Pretreatment Function

User Benefits

- Analysis of 20 proteinogenic amino acids can be performed in 26 minutes per cycle. - Pre-column derivatization analysis of amino acids can be easily performed by using the automatic pretreatment function of Nexera XR. - Nexera XR can also be used to analyze analytes besides amino acids.

Introduction

Amino acid analysis is required in various fields, including the development of food and drugs. Post-column derivatization method is commonly used for amino acid analysis with high performance liquid chromatography (HPLC), and Shimadzu also uses post-column derivatization method for Amino Acid Analysis System. However, it is difficult to realize high-speed analysis due to the characteristics of the column. The pre- column derivatization method, in which amino acids are derivatized before entering the column, enables high-speed analysis and simple derivatization procedure in comparison with the post-column derivatization method by automating the derivatization procedures. This article introduces an optimized analytical conditions of amino acids analysis by the automated pre-column derivatization method using HPLC (Nexera XR).

September 29, 2022 GMT