Quantitative Analysis of Pre-Column Derivatized Amino Acids by HPLC-Fluorescence Detector Using Automatic Pretreatment Function

User Benefits

- Quantitative analysis of amino acids can be performed with good repeatability. - Pre-column derivatization analysis of amino acids can be easily performed by using the automatic pretreatment function of Nexera XR. - Analysis of 20 proteinogenic amino acids can be performed in 24 minutes per cycle.

Introduction

Analysis of amino acids is considered necessary in a wide range of fields, such as development of food products and pharmaceuticals. Various techniques are used, including the HPLC-fluorescence detector, HPLC-UV detector, LC/MS, and GC/MS, taking advantage of the respective features of these instruments. The Application News 01-00007-EN introduced an analysis of amino acids by the automated pre-column derivatization method using HPLC-fluorescence detector. This article introduces an example in which an analysis method using those conditions was applied to a quantitative analysis of amino acids.

May 16, 2022 GMT