High Efficiency Method Scouting for Small Molecule Drugs

User Benefits

- Nexera Method Scouting System enables the labor-saving and improvement of efficiency in HPLC method development. - Complicated analytical conditions changes can be automated by simple operations. - The multi-data report function enables quantitative evaluation of scouting results and easy determination for the optimum conditions.

Introduction

The columns (stationary phase) and mobile phases used are parameters which have a large effect on retention and separation of target compounds in HPLC analysis. Conducting method scouting first to narrow the options of columns and mobile phases is an extremely effective approach for improving the efficiency of method development. However, in the process of method scouting, it is necessary to actually perform the analyses under various conditions in order to determine the optimum method for the target compounds from among the large number of possible mobile phases (buffer solution pH, salt concentration, organic solvent ratio) and columns (ODS, C8, phenyl). Moreover, considerable time, labor, and skill are also required in work such as the switching of columns and preparation/setting of the mobile phases. This article introduces the Nexera Method Scouting System and the dedicated software "Method Scouting Solution", which were developed to automate the method scouting workflow, through a example of scouting conditions for simultaneous analysis of small molecule drugs.

June 28, 2022 GMT