Analysis of Polyether Antibiotics in Animal Feeds Using Post-Column Derivatization

User Benefits

- Three polyether antibiotics can be stably quantified over a wide concentration range in accordance with "Feed Analysis Standards". - Rapid analysis can be performed with easy operation compared to the microbiological quantification method listed in "Feed Analysis Standards". - Significant labor-saving and improved efficiency of analytical procedure can be achieved by automated entire analytical process from instrument startup to shutdown.

Introduction

Polyether antibiotics are designated as feed additives in accordance with the Public Notice oNo.750 by the Japanese Ministry of Agriculture, Forestry and Fisheries (MAFF). For the analytical methods of these antibiotic components, microbiological quantification, HPLC, and LC/MS methods are specified in "Feed Analysis Standards," the Notice by the Director-General, Food Safety and Consumer Affairs Bureau, MAFF. While the microbiological quantification method requires long pretreatment such as culturing test bacteria for 16 to 24 hours, the HPLC method can quickly obtain results with an average pretreatment time of 20 minutes. This article introduces a analysis of salinomycin sodium (SL), monensin sodium (MN) and narasin (NR), specifically designated to apply post-column VIS photometric derivatization using HPLC.

June 28, 2022 GMT