Quantitative Determination of Fat-Soluble Vitamins in Infant Formula by LC-MS/MS Method with Supported Liquid Extraction (SLE)

User Benefits

- A LC-MS/MS method with sample preparation using supported liquid extraction (SLE) column is established for quantitative determination of fat-soluble vitamins (A, 1, D3, E and K1) in infant formula on LCMS-8045. - The reliability of the method was verified and confirmed with analysis of fat-soluble vitamins in FAPAS QC quality control material. The analysis results indicate the acceptable accuracy and precision of the vitamin analysis.

Introduction

Fat-soluble vitamins are classified into four categories: Vitamin A, D, E and K. They have important roles in several functions of the human body, such as vision (vitamin A), calcium absorption (Vitamin D), antioxidative protection in cell membranes (Vitamin E), and blood coagulation (Vitamin K). Infant formula, as a main source of feeding for infants, must be able to provide sufficient vitamins. Due to complexity and instability of Fat-soluble vitamins during extraction, their quantitation remains challenging. Thus, extraction is the critical step in determination of fat-soluble vitamins in milk samples. In this application notes, a sensitive MRM method is described for the quantitative determination of fat- soluble vitamins in infant formula with supported liquid extraction (SLE) on LCMS-8045, a tandem LC-MS/MS system with an APCI interface. *FAPAS: “Food analysis performance Assessment Scheme” is the proficiency scheme for food and water testing, organized by the Food and Environment Research Agency, UK.

October 17, 2022 GMT