A LC-ESI-Q-TOF Method for Identification and Relative Composition Analysis of Triacylglycerols in Tropical Oil -(3) Moringa OleiferaSeed Oil

User Benefits

- A fast and direct LC-ESI-QTOF method was established and adopted in identification and composition analysis of TAGs in moringa oleifera seed oil. A total of 33 TAGs was identified based on accurate mass MS and MS/MS spectra. - The result indicates that TAGs of moringa oleifera seed oil are mainly consisted of triolein (OOO, 39.7%) and OOX (X=P, S, A, B, Li, G) with ECN at 48~56. It is a high oleic acid containing oil.

Introduction

This research is completed in collaboration between Sinarmas Agribusiness & Food (R&D) Marunda Centre and Shimadzu Asia Pacific. Moringa oleifera tree is found widely in tropical and subtropical areas worldwide. Moringa oleifera seed oil has been used as nutritional supplement, traditional medicines, skin treatment oil and new food resource. Studies on properties of moringa oleifera seed oil rely on the analysis of the main components - triacylglycerols (TAGs), their identification and composition. The conventional analytical approach is to determine fatty acids of TAGs. It involves first hydrolysis of TAGs to form fatty acid methyl esters, followed by GC-FID analysis. In recent years, direct analysis of TAGs by LC-MS/MS and SFC-MS/MS have been developed and used for identification of TAGs in various edible oils. In this study, a LC-ESI-Q-TOF method established previously was applied in identification of TAGs based on accurate mass spectra and determination of relative composition of the TAGs in moringa oleifera seed oil samples.

December 13, 2022 GMT