Screening of Ethylene Glycol and Diethylene Glycol in Medicinal Syrup by GCMS with FASST mode (Part 2 – Improved Method for QC testing)

User Benefits

- GCMS-QP2020 NX delivers high scan speed capabilities for screening analysis - Fast Automated Scan/SIM Type (FASST) mode enables consecutive collection of scan and single ion monitoring (SIM) data - Accurately identify and quantify both EG and DEG in medicinal syrup with a single injection - Superior reliability, reproducibility and selectivity of the results obtained - Matrix-matched calibration greatly improves %Recovery

Introduction

In Part 1 of the application news, we have successfully demonstrated GCMS-QP2020 NX superior capability in screening of ethylene glycol (EG) and diethylene glycol (DEG) in medicinal syrup, using the recommended method by Indonesian BPOM. The method requires minimum sample preparation, making it suitable for laboratories in relevant authorities to screen medicinal syrup from different brands easily, thus making it possible to make rapid assessment on the safety of the medicinal syrup on the market. However, notable matrix effect has been observed, thus affecting the %Recovery of the method. As a result, this approach might not be capable of meeting the stringent QC testing requirements in pharmaceutical industry. Part 2 of the application news intends to address this gap. To improve the accuracy of the method, our team decided to modify the BPOM method slightly using matrix-matched calibration plot. This slight modification greatly enhances the method selectivity, enabling accurate quantitation of EG and DEG in the presence of complex sample matrices. In this article, we will be using the exact setup as Part 1. We will examine the usage of the Shimadzu GCMS-QP2020 NX to identify and quantify EG and DEG in medicinal syrup. The analysis will be demonstrated using the Fast Automated Scan/SIM Type (FASST) mode, which enables consecutive operation of scan mode and selected ion monitoring (SIM) mode for accurate qualification and quantitation within a single injection.

February 22, 2023 GMT