Highly-Efficient Screening Approach for Toxicological Compounds in Human Blood Samples on LCMS-9030

User Benefits

- Highly-efficient data analysis workflows for targeted and untargeted screening of toxicological compounds can be performed using the LabSolutions Insight Explore^TM – Analyze. - The workflow was applied to DDA and DIA data of spiked human blood samples acquired on LCMS-9030.

Introduction

Efficient multi-target and/or untargeted screening by LC- MS methods have been developed and used in detection and quantitation of drugs of abuse (DoA) in forensic and clinical toxicology. The screening analysis must enable to detect of a wide range of compounds including illicit drugs, narcotics, psychotropics, antipsychotics, pharmaceuticals and other toxic compounds in urine, serum/plasma and whole blood samples. High resolution mass spectrometer (HRMS) coupled with UHPLC has played key roles in such analysis due to the superior mass accuracy (1 ppm) and high MS/MS capacity by Data- Dependent Acquisition (DDA) or Data-Independent Acquisition (DIA) modes on Q-TOF system. The aim of this study is to demonstrate efficient data analysis approaches for screening analysis of toxicological compounds by DDA and DIA modes on LCMS-9030 Q- TOF system. The LabSolutions Insight Explore suite was used as data analysis tools for (1) multi-target screening; (2) HRMS library search for confirmation and (3) untargeted screening and unknown identification. Both DDA and DIA data were processed and analysed. Spiked samples in human blood with mixture of 61 drug standards were used in this work.

March 1, 2023 GMT