Quantitative Analysis of Mycophenolic Acid and Metabolites in Serum Using a Single Quadrupole Mass Spectrometer

User Benefits

- Mycophenolic acid and glucuronic acid conjugate can be analyzed in 2 minutes per sample using a single Q-MS. - The LCMS-2050 is compact, thus allowing it to be used in the same installation space as an HPLC instrument.

Introduction

Immunoassay is commonly used for measuring drug concentrations in biological samples. However, non-specific reactions and lot-to-lot differences in antibody reagents have been noted. Liquid chromatography is a way to overcome these problems. Diode array detectors and fluorescence detectors are the mainstays of LC. Still, they can require long analysis times due to separation from matrices, and sensitivity may also be insufficient. A mass spectrometer (MS) is highly selective because it is capable of separation according to m/z and is superior in terms of throughput and sensitivity. Therefore, LC/MS is now replacing immunoassay as the mainstream analysis method. This article presents an example of the analysis of mycophenolic acid (MPA) and glucuronic acid conjugate (MPA-G) in plasma using a single quadrupole mass spectrometer. Single quadrupole mass spectrometers are less expensive than triple quadrupole mass spectrometers and have simple condition settings, making it easy even for those new to mass spectrometry to begin an analysis.

March 1, 2023 GMT