Quantification of Six Porphyrin Biomarkers in Urine Using LC-2050C with Fluorescence Detection

User Benefits

- A simple and robust HPLC method for the determination of porphyrins in urine. - No sample pre-treatment is required.

Introduction

Porphyria are a group of seven metabolic disorders of haem biosynthesis. These include aminolevulinic acid dehydratase porphyria (ADP), acute intermittent porphyria (AIP), variegate porphyria (VP), hereditary coproporphyria (HCP), porphyria cutanea tarda (PCT), erythropoietic protoporphyria (EPP) and congenital erythropoietic porphyria (CEP). People with certain types of porphyria can have a sudden life-threatening crisis. These attacks are rare and difficult to diagnose because they are non-specific. In many cases, the disease is misdiagnosed because of the rarity of some types of porphyria. For early diagnosis and identification of the type of porphyria, accurate quantification of porphyrins in urine and faeces is necessary. This application note describes an HPLC method with fluorescence detection for the quantification of six porphyrin biomarkers; namely, uroporphyrin I, heptacarboxyporphyrin I, hexacarboxyporphyrin I, pentacarboxyporphyrin I, copro-porphyrin I and coproporphyrin III in urine.

August 5, 2024 GMT