Analysis of 3-MCPD Fatty Acid Esters and Glycidol Fatty Acid Esters in Palm Oil Using GC-MS/MS in Accordance with AOCS Method Cd 29c-13

User Benefits

- Sample preparation workflow in accordance with AOCS Cd 29c-13 Method - Highly sensitive 3-MCPD in palm oil analysis with LOQ of 20 ppb and calculated LOD of 6 ppb - Backflush system to discharge high boiling substances from the column

Introduction

Fatty-acid-bound 3–monochloropropane-1,2-diol or known as ester-bound 3-monochloropropanediol (3-MCPD fatty acid esters) and glycidyl fatty acid ester (GE) are processed contaminants generated mainly during the refining of edible oil. During the oil refining, often high temperature is required which causes 3-MCPD (fatty acid esters) and GE to be formed as by-products. Both 3-MCPD (fatty acid esters) and GE are possibly carcinogenic which has raised concern to human health if consumed at high levels. European Commission has recently amended Regulation (EC) No. 1881/2006 which regards to the maximum levels of 3-MCPD (fatty acid esters) and GE. The sum of 3-MCPD (fatty acid esters) expressed in 3-MCPD for edible oil should not exceed 1,250 μg/kg for oils and fats produced from coconut, maize, rapeseed, sunflower, soybean, palm kernel and olive oils (composed of refined olive oil and virgin olive oil) and mixtures of oils and fats with oils and fats from this category only. Meanwhile, the amount of GE expressed in glycidol should not exceed 1,000 μg/kg from oils and fats produced from vegetables, fish, and other marine organism. Among these, palm oil has the highest content of 3-MCPD (fatty acid esters) and GE due to the nature of refining process that require high temperature in the deodorization stage. Following this regulation, AOCS has developed official methods, namely Cd 29a-13, Cd 29b-13 and Cd 29c-13 to quantify 3- MCPD (fatty acid esters) and GE in edible oils and fats. In this application news, an MRM method using Shimadzu GCMS- TQ8050^TM NX was established and evaluated for quantitative determination of both 3-MCPD (fatty acid esters) and glycidol esters in palm oil expressed in their free-forms following Cd 29c- 13 method. Furthermore, reproducibility of the method was also evaluated.

July 6, 2023 GMT