Determination of Aminoglycoside Drugs Residual in Bee Products by LC-MS/MS

User Benefits

- Heptafluorobutyric acid is added to the injection vial to enhance the retention of aminoglycosides - There is no need to use ion-pairing reagents and highly concentrated salt solutions in the mobile phase, which might inhibit the mass spectrometry signal

Introduction

Aminoglycosides (AGs) are composed of glycoglycans and aminocyclic alcohols combined with glycoside bonds. An aminoglycoside's main role is to hinder the protein synthesis of bacteria, so the permeability of bacterial cell walls changes, which exerts antibacterial effects. In recent years, it has been reported that AGs have significant ototoxicity, nephrotoxicity, and vestibular function damage, which can lead to shock and even death in severe cases. GB 31650-2019 “Maximum Residue Limits of Veterinary Drugs in Food” stipulates residue limits of gentamicin, kanamycin, spectinomycin, streptomycin, dihydrostreptomycin, and neomycin B in different matrices. In this paper, a method for the detection of aminoglycoside residues in honey was established. The extract was divided into two equal parts and purified by MCX and WCX SPE cartridges, respectively. This method covers AGs commonly used in the livestock and poultry industry. Ion-pairing reagents and high concentration salt solutions are not needed in the mobile phase, and the results are accurate and reliable, and can effectively detect the residues of aminoglycosides in bee products.

February 17, 2025 GMT