Oligonucleotide Sequence Analysis Using MALDImini-1 and LabSolutions Insight Biologics Software

User Benefits

- The compact space-saving MALDImini-1 MALDI-DIT-MS system enables operation in facilities with limited space. - LabSolutions Insight Biologics software rapidly analyzes complex fragment spectra of oligonucleotides to reduce data analysis time. - The combination of the MALDImini-1 spectrometer and LabSolutions Insight Biologics software enables molecular weight and sequence analysis in approximately 10 minutes, from measurement to final results.

Introduction

With rapid advancements in the practical application of oligonucleotide therapeutics, there is an increasing need for effective analytical techniques for such applications. With mass spectrometry (MS) now being applied to the analysis of oligonucleotide therapeutics, simpler and faster analytical and sequencing techniques are increasingly desired. This Application News article describes an example of an oligonucleotide sequence analysis using the MALDImini-1 matrix-assisted laser desorption/ionization digital ion trap mass spectrometer (MALDI-DIT-MS) system in combination with LabSolutions Insight Biologics software for oligonucleotide sequence characterization. Due to the compact and space-saving size of the MALDImini-1 MALDI-DIT-MS system it can operate on a 100 V power supply in facilities with limited space. In addition, because it only generates singly-charged ion species, the LabSolutions Insight Biologics software can rapidly estimate the base sequence of the oligonucleotides, including modification information. Because the sequence analysis is limited to singly-charged fragment ions, the estimated sequences displayed are simple and contribute to the reliability of analysis results. Due to the compact space-saving design of the analytical system, the system offers rapid and simple molecular weight (MW) analysis and sequence analysis of oligonucleotides.

April 23, 2025 GMT