Automated Preparation of Calibration Curves Using the Autosampler's Dilution Function -Application to Organic Acid Analysis-

User Benefits

- The auto-dilution function equipped in the autosampler eliminates the need for manual dilution during calibration curve creation and sample preparation. - Samples can be automatically diluted to create a calibration curve by specifying the desired dilution factor in the batch table. - Because the same method file can be used across different dilution factors, only minor adjustments are needed when changing dilution conditions.

Introduction

In LC quantification, the dilution of standard and sample solutions is typically performed manually using pipettes, which is a labor-intensive and time-consuming process. Recent advancements have called for automation to reduce labor and improve efficiency. By automating the dilution process, workflows can be streamlined, leading to increased productivity. The Nexera series autosamplers are equipped with an automatic pretreatment function that enables automated dilution. Using the auto-dilution function, samples can be automatically prepared at any desired dilution factor and directly introduced into the analytical column. This article presents a method to automate the creation of calibration curves and subsequent quantification workflows using the autosampler’s auto-dilution function.

May 13, 2025 GMT