Simultaneous Analysis of CHO Cell Culture Supernatant Components Using a Single Quadrupole Mass Spectrometer

User Benefits

- A single quadrupole LC-MS system can be used to simultaneously analyze amino acids, organic acids, vitamins, and other substances in a culture supernatant. - Changes in the components in a culture supernatant can be easily visualized as a function of time by analyzing culture supernatant samples acquired over time with the Multi-omics Analysis Package.

Introduction

When using cells to produce beneficial substances or manufacture antibody drugs, the pH level of the culture medium, dissolved gases, carbon sources, nitrogen sources, and other factors are monitored to optimize or manage the culturing process. Cell cultures can contain a variety of substances, including not only glucose and glutamine but also vitamins, nucleic acid-related compounds, and metabolites secreted from cells. Therefore, by comprehensively analyzing all the components in a culture medium, useful information about biological processes can be obtained. This Application News article describes using a single quadrupole LC-MS system to monitor time-course changes in the components in a supernatant from culturing CHO-K1 cells, which are commonly used for antibody production, by simultaneously analyzing all the components. Because single quadrupole LC-MS systems have a simple configuration process, it is easy even for first-time users to simultaneously analyze components in culture supernatants.

June 3, 2025 GMT