Enhancing Efficiency of Method Development with Integrated LC System

User Benefits

- A comprehensive analysis schedule can be automatically generated by varying LC parameters such as gradient conditions and column oven temperatures. - By utilizing Evaluation Value provided by LabSolutions MD, optimal separation conditions can be quickly identified from a large amount of data.

Introduction

Developing appropriate separation conditions in LC method development often requires considerable time. For example, creating an analysis schedule to explore a wide range of conditions can be a labor-intensive process. In addition, evaluating which conditions provide the desired separation requires reviewing all chromatograms obtained, resulting in significant effort for data analysis. This article introduces an approach for simplifying and accelerating the process of optimization of separation conditions by combining the integrated LC system, i-Series, with LabSolutions MD, a dedicated software for supporting method development. Specifically, a mixture of nine small-molecule compounds was used as a model sample, and an analysis schedule was automatically generated to systematically vary parameters such as gradient conditions and column oven temperatures. Furthermore, Evaluation Value calculated by LabSolutions MD were applied to the large volume of acquired data, enabling the rapid identification of optimal separation conditions.

August 31, 2025 GMT