Analysis of Saccharides Using Integrated HPLC and Size Exclusion-Ligand Exchange (Na Type) Column

User Benefits

- Saccharides contained in fruit juices can be analyzed simultaneously. - There is no need to prepare the mobile phase since analysis can be done using only water as mobile phase. - The integrated HPLC system allows for the installation of a refractive index detector without changing the footprint.

Introduction

Saccharides are the main component of sweetness in food and an important source of energy. However, excessive saccharide intake has been reported to increase the risk of lifestyle-related diseases such as obesity and dental caries1). Monitoring the amount of saccharide in food plays an important role in quality control and nutritional management. Saccharides have almost no ultraviolet absorption, so they are detected using differential refractive index detector, evaporative light scattering detector, a mass spectrometer, etc. Our integrated HPLC can be connected with these detectors. High column oven temperature such as around 80 ˚Crequired in size exclusion -ligand exchange mode is available because upperlimit of controllable temperature is 90 ˚C. In this article, saccharide analysis in fruit juice using an integrated HPLC and differential refractive index detector is introduced.

August 31, 2025 GMT