Evaluation of Tissue-Specific Plant Metabolites Using Probe Electrospray Ionization Kit and LC-MS

User Benefits

- Intuitive operation allows for the simple detection of metabolites from complex and minute parts of plant tissue. - Enables high-throughput analysis since metabolites adhering to the probe are ionized directly, and column separation is unnecessary. - Applicable to various functional components.

Introduction

Plants contain numerous components related to color, taste, and functionality, which exhibit their functionality by being localized in specific parts of the plant. Detecting each component from the complex and minute parts of plants with high sensitivity requires many samples and time. Thus, there is a demand for simple and rapid analytical systems for micro-regions. So far, a detection system for anthocyanins in plants using probe electrospray ionization (PESI) and triple quadrupole LC-MS has been developed, demonstrating the utility of PESI in plant analysis. In PESI, the probe moves up and down while voltage is applied to the tip, ionizing the sample adhered to the probe surface and directly introducing it into the mass spectrometer. This article introduces an analytical method focused on localizing plant metabolites using PESI and quadrupole time-of-flight (QTOF) mass spectrometer. This method allows for intuitive and rapid sampling with the probe while enabling accurate mass spectrometry with TOF.

September 24, 2025 GMT