Maximizing Operational Productivity through Ultra-Fast Analysis with Nexera X4

User Benefits

- The low internal volume design of Nexera X4 significantly improves solvent delivery responsiveness and shortens re-equilibration time in gradient elution, enabling high-throughput analysis. - The independently actuated plungers and the pressure feedback mechanism incorporated into the solvent delivery pump ensure excellent reproducibility of both retention times and peak areas.

Introduction

In multi-sample analyses in pharmaceutical development, improving throughput per analysis directly translates to enhanced productivity. Nexera X4 is a next-generation ultra-high-performance liquid chromatograph (UHPLC) that inherits the technologies cultivated in Shimadzu’s Nexera series and delivers best-in-class analytical performance. Its low-internal-volume design significantly improves solvent delivery responsiveness, enabling ultra-fast analysis. In addition, the solvent delivery pump equipped with independently actuated plungers and a pressure feedback mechanism greatly reduces pulsation and provides highly stable and reproducible solvent delivery even under ultra-high-pressure conditions. All high-pressure sample flow paths employ end-surface-sealed connections that minimize carryover and peak broadening originating from voids (dead volume) in the fittings. Tool-free fittings are used throughout these flow paths to enhance usability. In this article, data are presented demonstrating how the superior solvent delivery responsiveness of Nexera X4 reduces cycle times compared with existing UHPLC systems and enables high-throughput analysis.

February 24, 2026 GMT