Achieving Sharp Peaks and High Sensitivity with Nexera X4

User Benefits

- The low-dispersion design of Nexera X4 suppresses extra-column band broadening within the system, enabling the column to deliver its maximum separation performance. This also results in sharper peak shapes and improved detection limits for impurity analysis.

Introduction

In high-performance liquid chromatography, minimizing sample band broadening both inside and outside the column is essential to achieving high separation performance. For example, impurity analysis often requires the separation of structurally similar components, demanding high separation performance and high sensitivity. Nexera X4 is a next-generation UHPLC system that inherits the advanced technologies of Shimadzu’s Nexera series and delivers top-class analytical performance. Its industry-leading low-dispersion design provides exceptionally sharp peaks and outstanding separation capability. In addition, Nexflow technology achieves low dispersion without changing the inner diameter of the flow lines, thereby minimizing system-derived band broadening while reducing the risk of tube blockage. This allows UHPLC columns to perform at their full potential and enables highly efficient separations. All high-pressure flow lines that samples pass through employ end-surface-sealed connections, minimizing dead volume–induced peak broadening. Tool-free fittings are also used to enhance usability. This article introduces an example in which impurities were separated with high resolution and high sensitivity using Nexera X4.

February 24, 2026 GMT